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’Phytoantibodies’: a general vector for the expression of immunoglobulin domains in transgenic plants

Titolo’Phytoantibodies’: a general vector for the expression of immunoglobulin domains in transgenic plants
Tipo di pubblicazioneArticolo su Rivista peer-reviewed
Anno di Pubblicazione1991
AutoriBenvenuto, Eugenio, Ordàs R.J., Tavazza Raffaela, Ancora Giorgio, Biocca S., Cattaneo A., and Galeffi Patrizia
RivistaPlant Molecular Biology
Volume17
Paginazione865 - 874
Data di pubblicazione1991///
Parole chiaveisolated VH domain (IVD), transgenic plants, ’single domain antibody’ (dAb)
Abstract

Sequences encoding the immunoglobulin heavy-chain variable (VH) domains were engineered in a new general purpose vector to transform plants via Agrobacterium. The expression of an isolated VH domain (IVD) after introduction into the plant genome has been monitored by northern, western and immuno-histochemical analysis. Immunoblotting showed that the polypeptide was stably expressed and accounted for up to 1% of the soluble protein fraction. It is therefore proposed that single immunoglobulin domains of suitable specificity expressed in plants may constitute an effective system to inhibit the activity of molecules involved in plant pathology or plant development. © 1991 Kluwer Academic Publishers.

Note

Cited By (since 1996): 55Export Date: 26 August 2010Source: Scopus

URLhttp://www.scopus.com/inward/record.url?eid=2-s2.0-0026245101&partnerID=40&md5=dc6b87949f09b26f9695a77dc122a566
Citation Key485