Title | ’Phytoantibodies’: a general vector for the expression of immunoglobulin domains in transgenic plants |
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Publication Type | Articolo su Rivista peer-reviewed |
Year of Publication | 1991 |
Authors | Benvenuto, Eugenio, Ordàs R.J., Tavazza Raffaela, Ancora Giorgio, Biocca S., Cattaneo A., and Galeffi Patrizia |
Journal | Plant Molecular Biology |
Volume | 17 |
Pagination | 865 - 874 |
Date Published | 1991/// |
Keywords | isolated VH domain (IVD), transgenic plants, ’single domain antibody’ (dAb) |
Abstract | Sequences encoding the immunoglobulin heavy-chain variable (VH) domains were engineered in a new general purpose vector to transform plants via Agrobacterium. The expression of an isolated VH domain (IVD) after introduction into the plant genome has been monitored by northern, western and immuno-histochemical analysis. Immunoblotting showed that the polypeptide was stably expressed and accounted for up to 1% of the soluble protein fraction. It is therefore proposed that single immunoglobulin domains of suitable specificity expressed in plants may constitute an effective system to inhibit the activity of molecules involved in plant pathology or plant development. © 1991 Kluwer Academic Publishers. |
Notes | Cited By (since 1996): 55Export Date: 26 August 2010Source: Scopus |
URL | http://www.scopus.com/inward/record.url?eid=2-s2.0-0026245101&partnerID=40&md5=dc6b87949f09b26f9695a77dc122a566 |
Citation Key | 485 |