Bioprospecting, pathway discovery, and metabolic engineering are three interconnected approaches that play a pivotal role in the production and valorization of high-value molecules. Acting synergistically, they represent a powerful biotechnological strategy to elucidate the enzymes and mechanisms underlying the accumulation of molecules in plant species. This enables the identification of new sources of target compounds and the optimization of their production through metabolic engineering, transforming natural resources into innovative solutions while promoting sustainability and the efficient use of biodiversity.