Metabolomics represents a key tool for the study of natural substances, enabling the analysis of their complex chemical composition and the identification of compounds of interest. The approach combines mass spectrometry with advanced bioinformatics tools to obtain a detailed characterization of the metabolites present in natural extracts. This allows for the selection of the most promising molecules for pharmaceutical, nutraceutical, and cosmetic applications. By analyzing metabolic profiles, this approach contributes to the valorization of biodiversity and the development of new, sustainable products based on naturally derived active ingredients.