This paper presents the instrumentation and use of a novel electroporation generator, configured of MOSFETs in a push-pull configuration, with an artificial 50 Omega buffer for possible real-time neutralization of cancer stem cells. The results from an initial bench study investigates the development of an electroporation generator capable of delivering non-thermal treatment with an original cuvette housing unit and its effects on isolated cancer stem cell. Initial permeabilization investigation of cancer stem cells were conducted, indicating that the developed protocols and devices have a strong potential future use in achieving electro-manipulation of cancer stem cells. © 2018 European Microwave Association.