| Title | Fecal HMGB1 is a novel marker of intestinal mucosal inflammation in pediatric inflammatory bowel disease |
|---|---|
| Publication Type | Articolo su Rivista peer-reviewed |
| Year of Publication | 2011 |
| Authors | Vitali, Roberta, Stronati L., Negroni Anna, Di Nardo G., Pierdomenico Maria, Del Giudice E., Rossi P., and Cucchiara S. |
| Journal | American Journal of Gastroenterology |
| Volume | 106 |
| Pagination | 2029-2040 |
| Keywords | Active Transport, adolescent, adult, article, Biological Markers, Biopsy, Caco-2 Cells, calgranulin, Cell Nucleus, cellular distribution, Child, clinical article, colitis, colonoscopy, controlled study, Crohn disease, cytoplasm, enteritis, feces, feces analysis, feces level, Female, Gene expression, high mobility group B1 protein, HMGB1 Protein, human, human cell, Humans, immunohistochemistry, Infant, Interferon-alpha, Interferon-gamma, Interleukin-8, Intestinal Mucosa, intestine biopsy, intestine mucosa, male, Messenger, messenger RNA, Preschool, priority journal, protein analysis, protein expression, protein function, protein localization, real time polymerase chain reaction, RNA, school child, Ulcerative, ulcerative colitis, Western blotting |
| Abstract | Objectives: High-mobility group box 1 (HMGB1) is a nuclear protein with functions in the regulation of transcription. In inflammatory conditions, HMGB1 is actively secreted from immune cells in the extracellular matrix, where it behaves as a proinflammatory cytokine. The aim of the present study was to investigate the role of HMGB1 in pediatric inflammatory bowel disease (IBD). Methods: We analyzed the stools of 19 children with Crohn's disease (CD), 21 with ulcerative colitis (UC), and 13 controls. The gene/protein expression levels of HMGB1 were assessed in bioptic specimens of all children using real-time PCR and western blot assay. Finally, intracellular localization of the protein was analyzed by western blot, after separation of nuclear and cytoplasmic extracts, and by immunohistochemistry. Results: HMGB1 protein levels were significantly increased (P<0.001) in the stools of patients, but were undetectable in the controls; fecal HMGB1 correlated well with fecal calprotectin levels (r: 0.77 in CD, r: 0.70 in UC; P<0.01); and mRNA and protein expression were unchanged in inflamed bioptic tissues compared with controls. However, by separately analyzing the nuclear and cytoplasmic fraction, we detected the cytoplasmic HMGB1 expression to be significantly enhanced (P<0.01) in the inflamed tissues of the patients. In addition, HMGB1 was significantly detected in 16 patients with inactive disease, whose endoscopic scores showed persisting inflammation, suggesting that it may be a sensitive marker of mucosal inflammation, although the disease is clinically inactive. Conclusions: It was shown for the first time in our study that HMGB1 is secreted by human inflamed intestinal tissues and abundantly found in the stools of IBD patients. Hence, it can be considered as a novel marker for intestinal inflammation. We can also suggest that the presence of HMGB1 in large amounts in the fecal stream of IBD patients is mainly due to active secretion of the protein stored in the nucleus rather than a de novo synthesis. © 2011 by the American College of Gastroenterology. |
| Notes | cited By 47 |
| URL | https://www.scopus.com/inward/record.uri?eid=2-s2.0-80655127791&doi=10.1038%2fajg.2011.231&partnerID=40&md5=93da03bf62f0b5935e69dc9f51c3fe3c |
| DOI | 10.1038/ajg.2011.231 |
| Citation Key | Vitali20112029 |
