The production of recombinant antibodies in plant systems has reached a high level of maturity, emerging as an effective and advantageous alternative to classical methods based on animal cell cultures. Key benefits include greater versatility in design and production, significantly reduced analysis times, lower costs, and improved reliability and robustness of analytical methods. Monoclonal antibodies specific for aflatoxins B1 and M1 have been isolated and produced in plants to develop “green” immunoassays for quantification in food matrices such as corn and milk.