The need for non-invasive techniques for clinical diagnosis remains an ongoing challenge. To identify innovative biomarkers and develop diagnostic kits for clinical use, we integrate advanced methodologies and multidisciplinary expertise. Our approach combines various omics analyses, bioinformatics tools, and analytical techniques to identify and validate diagnostic, prognostic, and disease monitoring biomarkers. These tools are essential not only for diagnosis but also for assessing disease progression and response to therapy, particularly in high-social impact contexts. The ultimate goal is to develop sustainable prototypes for clinical use that improve patients’ quality of life, promote personalized medicine, and optimize costs for the National Health Service.