Microfluidic systems require exact control of fluids at the microscale, crucial for applications including biomedical diagnostics and chemical synthesis. Conventional microvalve technologies frequently rely on mechanical components or external stimuli, complicating device architecture and impeding scaling. Photomobile polymers (PMPs)-materials that modify their structure when exposed to particular wavelengths of light-offer a promising option for fluid regulation in microfluidic devices. This research investigates the incorporation of PMPs into microfluidic channels to serve as lightresponsive valves. The objective is to utilize the photomechanical features of PMPs to attain non-invasive, remote, and accurate regulation of fluid flow, eliminating the necessity for intricate mechanical actuators or electrical inputs. Our approach follows advanced manufacturing methods that involve integrating PMP components into microchannels and analyzing their deformation response to regulated light stimulation. The capability to adjust the responsiveness of PMP valves via material composition and light factors is also examined. Utilizing PMP-based valves could markedly improve the functionality and adaptability of microfluidic systems, facilitating dynamic flow regulation for lab-on-A-chip devices, responsive sensors, and adjustable reaction conditions. This method facilitates the simplification of device manufacture and operation, hence enabling more accessible and versatile microfluidic technologies. This study's insights intend to enhance the design of smart microfluidic components and establish a basis for future research on light-Activated fluidic systems. © 2025 Elsevier B.V., All rights reserved.